“两次打击”急性肺损伤大鼠肺组织NF-kB p65 mRNA的表达

The Expression of NF-kB p65 mRNA in Lung Tissue of Acute Lung Injury Caused by 'Two Hits' in Rat

目的观察用油酸(OA)和脂多糖(LPS)两次打击急性肺损伤大鼠肺组织核因子———kB(NF_kB)p65 mRNA表达量的变化,探讨转录调节因子NF_kB p65与急性肺损伤的关系。方法用OA(0.2mL/kg)和LPS(2mg/kg)两次打击,建立两次打击急性肺损伤(ALI)模型,进行ALI大鼠血液白细胞(WBC)分类计数和肺指数测定、肺组织病理学检查,采用原位杂交检测肺组织中NF_kB p65 mRNA的表达。结果两次打击后的ALI大鼠血液中WBC和肺指数显著升高,WBC分类呈现淋巴细胞比率降低而中性粒细胞比例增高的变化,呈典型的急性炎症表现,肺脏病理改变严重。原位杂交检测显示,ALI大鼠肺组织细胞中NF_kB p65 mRNA的表达明显增强。结论在ALI大鼠肺组织细胞中NF_kB p65 mRNA的表达量增强,参与了急性肺损伤的发病作用。

Objective To observe the change of nuclear factor-kappa B（NF-kB） p65 mRNA expression in the lung tissue with acute lung injury （ALI） induced by oleic acid （OA） and lipopolysaccharide （LPS） in rats, and to investigate the relationship between nuclear factor kappa B p65 and ALI. Methods OA（0.2ml/kg） and LPS （2mg/kg） were injected intravenously to Wistar rats to develop ALI. The lung index （LI）, WBC count, and percentage of ploymorphonuclear leucocyte （PMN） were observed, and the expression level of NF-kB p65 mRNA in the lung tissue with ALI was determined by situ hybridization technique. Results WBC in peripheral blood, and lung index of the rats with ALI caused by the ‘two hits＇ were significantly increased, and PMN percentage changed markedly with the significant decrease in neutrophils and the significant increase in lymphocytes. The pathological sections of the lung showed an inflammatory change. The expression of NF-KB p65 mRNA in the lung tissue of the rots with ALI was markedly enhanced. Conclusion The enhanced expression of NF-kB p65 mRNA may make important contributions to development of ALI by ‘Two hits＇.