山茱萸总萜的降血糖作用

Hypoglycemic Effect of Terpenes from Fructus Corni

目的:通过正常小鼠、四氧嘧啶糖尿病模型小鼠和链脲佐菌素糖尿病模型大鼠研究山茱萸总萜的降血糖作用。方法:1.山茱萸总萜以100 mg.kg-1,50 mg.kg-1,25 mg.kg-1的剂量连续10 d灌胃给予正常小鼠,观察其降血糖作用。2.以相同剂量连续4 d灌胃给予正常小鼠,末次给药1 h后灌胃给予淀粉5 g/kg,观察其对糖耐量的影响。3.以相同剂量连续12 d灌胃给予四氧嘧啶糖尿病模型小鼠,观察其降血糖作用及对胰岛素水平的影响。4.以60 mg.kg-1,30 mg.kg-1,15 mg.kg-1的剂量连续14 d灌胃给予链脲佐菌素糖尿病模型大鼠,观察其降血糖作用及对胰岛素水平,糖化血清蛋白GSP和肝糖原含量的影响。结果:山茱萸总萜对正常小鼠的血糖水平无明显影响;100 mg.kg-1可提高正常小鼠的糖耐量(P<0.01);三个剂量均可显著降低四氧嘧啶糖尿病模型小鼠的血糖值(P<0.05),提高血清胰岛素水平(P<0.05);显著降低链脲佐菌素糖尿病模型大鼠的血糖值(P<0.05或0.01),增加肝糖原含量(P<0.05);60 mg.kg-1,30 mg.kg-1可以显著降低糖化血清蛋白GSP的含量(P<0.05)。结论:山茱萸总萜对糖尿病模型动物具有良好的降血糖作用。

AIM：To investigate the hypoglycemic effect of terpenes of Fructus Corni （TFC） on animal models of diabetes mellitus. METHODS： The normal mice received a daily intra-gastric dosage of 100 mg·kg^-1, 50 mg· kg^-1 and 25 mg· kg^-1 for 10 days, then the fasting serum glucose （FSG） was detected. The normal mice received the same dose of TFC for 4 days and were given starch（5 g·kg^-1） after last administration, then the sentm glucose（SG） was detected. The alloxan-induced diabetes mice were given the same dose of TFC for 12 days, the FSG and fasting insulin were detected. The streptozotocin-induced diabetes rats were given intra-gastric TFC at the doses of 60 mg·kg ^-1, 30 mg· kg^-1 and 15 mg·kg^-1 for 14 days, and the FSG, fasting insulin, glycosylated serum protein and hepatic glycogen were detected. RESULTS： The glucose tolerance at 100 mg· kg^-1 in normal mice was significantly increased （P 〈 0.01 ）, but no significant decrease of FSG level was observed at all the three doses. It was observable on animals after treatment of all the three doses, that the FSG level decreased significantly（ P 〈 0.05）and the fasting insulin level elevated（ P 〈 0.05） in alloxan-induced diabetes mice, while FSG level also decreased significantly and the hepatic glycogen content increased in streptozotocin-induced diabetes rats. The doses of 60 mg· kg^-1 and 30 mg· kg^-1 could evidently alleviate the level of glycosylated serum protein（ P 〈 0.05）. CONCLUSION：Terpenes of Fructus Corni has remarkable hypoglycemic effect on diabetes animal models.