槐角中的抗癌活性成分

The Anticancer Activity in vitro of Constituents from Fruits of Sophora japonica

AIM: To study the isoflavonoids and their glycosides from the fruits of Sophora japonica L.and to investigate the anticancer activity in vitro of these compounds.METHOD: The constituents were isolated and purified by chromatographic techniques and their structures were elucidated by physico-chemical evidence and spectra data.The inhibitory activity on cultured human cancer cell lines was determined by MTT assay.RESULTS: Nine compounds were isolated and identified as:genistein（Ⅰ）,sophororicoside（Ⅱ）,genistin（Ⅲ）,sophorabioside（Ⅳ）,genistein 7,4′-di-O-βD-glucoside（Ⅴ）,formononetin（Ⅵ）,ononin（Ⅶ）,daidzin（Ⅷ） and afrormosin （Ⅸ）.Results showed that tumor inhibitory rate of compounds Ⅰ～Ⅲ on(100 Hg·mL-1) A549 reached 82.01%,38.87% and 32.97% respectively,and on BGC-823 reached 91.25%,23.26% and 13.98% respectively.CONCLUSION: Compounds Ⅶ and Ⅷ were isolated from this plant for the first time;compound Ⅸ was isolated from the fruits of the plant for the first time.The study showes that genistein has inhibitory effect on A549 and BGC-823 cell in vitro.

AIM： To study the isoflavonoids and their glycosides from the fruits of Sophora japonica L, and to investigate the anticancer activity in vitro of these compounds. METHOD： The constituents were isolated and purified by chromatographic techniques and their stuctures were clucidated by physico-chemical evidence and spectra data. The inhibitory activity on cultured human cancer cell lines was determined by MTT ashy. RESULTS： Nine compounds were isolated and identified as： genistein （ Ⅰ ）, sophororicoside （ Ⅱ）, genistin （Ⅲ） sophorabloside （ Ⅳ ）, genistein 7,4′-di- O-β-D-glucoside （ Ⅴ ）, formononetin （ Ⅵ）, ononin （ Ⅶ ）, daidzin （ Ⅶ） and afronnosin （Ⅸ） Resulls showed that tumor inhibitory rate of compounds Ⅰ- Ⅲ on （100 Hg·mL^-1） A549 reached 82.01%, 38.87% and 32.97% respectively and on BGC-832 reached 91.25%,23.26%and13.98% respectively.CONCLUSION：Compounds Ⅶ and Ⅷ were isolated from this plant for the first time; compound Ⅸ was isolated from the fruits of the plant for the first time. The study showes that genisitein has inhibitory effect on A549 and BGC-823 cell in vitro.