摘要
目的:观察外源性给予改构体酸性成纤维细胞生长因子(acid fibrob last growth factor,aFGF)后,大鼠小肠功能指标的变化规律,探讨改构体aFGF对肠缺血-再灌注损伤的影响。方法:以大鼠肠系膜上动脉(SM A)夹闭造成肠缺血-再灌注损伤模型,并将动物随机分为假手术组、生理盐水对照组、改构体aFGF治疗组和野生型aFGF治疗组。除假手术组外,其余各组动物均于缺血45 m in后于2、6、12、24 h活杀,取血及小肠组织标本,检测血浆中D-乳酸含量及组织中增殖细胞核抗原(PCNA)的表达特性。结果:血浆D-乳酸变化及HE染色显示,再灌注后6 h屏障功能损伤最严重,而改构体aFGF治疗组损伤在伤后24 h较生理盐水对照组有所减轻。PCNA的表达趋势与D-乳酸类似。结论:改构体aFGF对肠缺血-再灌注损伤具有一定保护与促修复作用。
Objective： To identify the regulation of change of the plasma D--lactate in rat intestine after the administration of acidic fibroblast growth factor （aFGF）, to explore the effects of fibroblast growth factor on the gut ischemia--reperfusion （I/R） injury in rats. Methods： Rats I/R injury was produced by clamping the superior mesenteric artery （SMA）, and the animals were divided randomly into sham--operation group, normal saline control group, reconstructive human aFGF treatment group, wild type aFGF treatment group. Blood samples and tissue specimens were collected at 2, 6, 12, and 24 hours after reperfusion. The levels of plasma D--lactate and the expression of proliferating cell nuclear antigen （PCNA） were detected. Results： The high activity of plasma D--lactate and pathological changes indicated that the intestinal barrier was damaged seriously 6 hours after reperfusion. However, the improvement in histological structures was observed 24 hours after reperfusion in reconstructive human aFGF treatment group and wild type aFGF treatment group. Conclusion： Reconstructive human aFGF may play an important role in intestinal wound healing after injury.
出处
《内蒙古医学院学报》
2005年第4期303-306,共4页
Acta Academiae Medicinae Neimongol
基金
国家重大基础研究规划资助项目(G1999054204)
国家自然科学基金重点项目(30230370)
基金项目(30170966)
国家863资助项目(2001AA215131)
