高效液相色谱法同时测定胆汁、胰液中的加替沙星、左氧氟沙星和奥硝唑

Determination of Gatifloxacin,Levofloxacin and Ornidazole in Bile and Pancreatic Juice by RP-HPLC

目的 建立RP-HPLC测定胆汁与胰液中加替沙星、左氧氟沙星和奥硝唑浓度的方法。方法 以乙腈-0．3％磷酸（16：84，三乙胺调pH为2．5）为流动相，流速为1．0mL·min^-1检测波长286nm。结果 加替沙星、左氧氟沙星、奥硝唑质量浓度分别在0．064～4．80，0．064～4．80，0．16～12．80mg·L^-1内线性关系良好，有较好的回收率与精密度。结论 本法适用于对胆汁与胰液中该3种药物的监测。

OBJECTIVE To establish a RP-HPLC method for the determination of gatifloxacin,levofloxacin and omidazole in human bile and pancreatic juice. METHODS The mobile phase was acetonitrile-0.3% phosphoric acid（ 16：84, pH 2.5 adjusted by triethylamine）.The flow rate was 1.0 mL· min^-1. The wavelength of UV detection was 286 nm. RESULTS The linearity was obtained in the rang of 0.064 4.80 mg·L^-1 of gatifloxacin, 0. 064 - 4.80 mg·L^-1of levofloxacin and 0.16 - 12.80 mg· L^-1 of ornidazole. The average recovery and relative standard deviation were good. CONCLUSION The established method can be used for the determination of the drugs mentioned in bile and pancreatic juice.