丹参对持续癫癎幼鼠脑损伤保护作用的实验研究

Protective effect of Salvia miltiorrhiza Bge against seizure-induced brain injury in young rats

目的探讨丹参对持续癫癎发作诱发幼鼠脑神经元损伤是否具有保护作用。方法皮下及腹腔注射贝美格针诱发健康幼龄鼠癫癎持续状态发作。光镜下观察神经元病变情况;电镜观察海马神经元超微结构的改变。结果持续癫癎组幼鼠脑组织光镜下可见明显的神经元病变,电镜下可见海马区神经元的超微结构病变。丹参治疗组神经元病变均轻于持续癫癎组;而正常对照组未见类似病变。结论丹参在组织、细胞和亚细胞水平对持续癫癎幼鼠脑神经元损伤具有一定的保护作用,为临床有效防治小儿惊厥性脑损伤提供了可靠的实验依据。

Objective To evaluate the protective effect of Salvia miltiorrhiza Bge（SMB） against seizureinduced cerebral neuron injury in young rats.Methods Megimide（20 mg/kg） was injected to young rats to evoke status epilepticus. Lsions in hippocampal neurons were observed by optical microscopy. The ultrastructural changes were observed under the electronic microscope.Results In the control group, the lesions in hippocampus neurons were apparent after the seizures and the mitochondria decreased, whereas the SMB group had as lighter damage than the control group. Conclusions Salvia miltiorrhiza Bge has a positive effect on the seizure-induced brain neuron injuries in young rats, which provides the basis for the clinical treatment of seizure - induced brain injury in humans.