燃煤型氟骨症早期大鼠血清IGF-1和尿HYP变化研究

STUDY ON THE CHANGE OF INSULIN LIKE GROWTH FACTOR-1(IGF-1)IN SERUM AND HYP IN URINE AT THE EARLY PHASE OF SKELETAL FLUOROSIS CAUSED BY FLUORIDE IN COAL.

目的:研究燃煤型氟中毒骨病变早期血清IGF-1和尿HYP的变化。方法:以SD大鼠为实验对象,随机分6组(组内雌雄各半):对照、低氟、中氟加营养、中氟、高氟加营养、高氟组。各染毒组喂饲含不同比例的燃煤型病区煤烘玉米的饲料复制燃煤型氟中毒动物模型。分两批以股动脉放血法处死动物,查看氟斑牙,测尿、骨、肾氟,骨密度(BMD),骨Ca、尿Ca,血清胰岛素样生长因子-1(IGF-1)。结果:①建成氟中毒动物模型各染毒组均出现氟中毒,对照组正常。中毒严重程度随氟剂量增加而加重;氟剂量相同时,营养好,中毒程度轻。②IGF-1早期中营组较低氟组升高(P<0.05),后期除高营组外,各染毒组血清IGF-1有上升趋势。说明接触一段时间后,染氟大鼠骨形成加速,氟通过IGF-1对骨形成的促进作用较晚,且受氟剂量及营养状况影响。③对胶原代谢产物(12 h尿HYP)的影响:染毒组有上升趋势,后期高剂量组升高有意义(P<0.05或0.01),且高氟组高于其余各组(P<0.05或0.01),高剂量组后期较早期高(P<0.05或0.01)。表明:随染氟剂量增加和接触时间延长,氟对胶原的损伤加重。结论:①尚不能认为血清IGF-1是反映燃煤型氟骨症病情的一个早期指标;②降低摄氟量及改善营养状况,可缓解氟中毒病情。

Objective： To study the change of insulin like growth factor- 1 （IGF- 1） in serum and HYP in urine at the early phase of skeletal fluorosis caused by fluoride in coal.Methods： SD rats were taken as the experimental objects, being randomly assorted into 6 groups （the number either female or male was same in each group） ： control group, low- dose fluoride group, middledose fluoride with added nutrition group, middle- dose fluoride group, high - dose fluoride with added nutrition group, high - dose fluoride group. All rats were killed by means of arteria femoralis phlebotomy, examined dental fluorosis, fluorine in urine, bone and kidney, hone mineral density （BMD）, calcium in bone and urine, IGF- 1 in serum.Results： ①The animal model of fluorosis was successfully established. There were fluorosis in every test group, but rots in the control group were all right. The higher the fluoride dose was, the more serious fluoresis syndrome of the rats were. On the condition of same fluoride dose, the fluorosis syndrome was mild if nutrition was good. ②At the earlier phase, IGF - 1 in serum of the middle - dose with nutrition group was higher than that of the low-dose group （P〈0.05）; at the latter phase, except the high-dose with nutrition group, IGF- 1 of every test group was higher than that of the control group, But there was no statistical significance between them. It indicated that fluoride, promoted skeletal formation after rats＇ being administered fluoride but it was very late that it affecting skeletal formation through IGF- 1. And the procedure was influenced by fluoride dose and nutritional status. ③The effect of coal - burning - fluorosis on collagen metaholite （12 hours HYP in urine） HYP of every test group had a increase trend; at the latter phase, HYP of the high- dose and nutrition group and the high - dose with nutrition group were higher than that of the control group （P〈 0.05 or 0.01）, HYP of the high- dose with nutrition group was higher than that of the other test groups （ P 〈 0.05 or 0.01） ; HYP of the high - dose with added nutrition group and the high - dose groups at the latter phase were higher than that at the earlier phase （P 〈 0.05 or 0.01）. It indicated that the higher the fluoride dose and the longer the experimental time were, the more serious harm that fluoride did to collagen.Conclusions： ①IGF- 1 couldn＇t be taken as an earlier index of skeletal fluorosis caused by fluoride in coal by far.②Reducing fluoride intake and improving nutritional status can release sydromes of fluorosis.