摘要
根据猪链球菌2型(strep tococcus su is type 2)溶血素基因(sly)设计和合成了一对可扩增其完整阅读框的引物,对HA 9801等6株猪链球菌2型江苏分离株、1株德国分离株SS2-D及猪链球菌C群参考株ATCC 35246的核酸进行PCR扩增,结果显示HA 9801等6株江苏分离株及德国株SS2呈阳性,ATCC 35246呈阴性。HA 9801株PCR产物纯化后测序,序列分析结果表明该DNA片段与猪链球菌2型1933株的sly基因同源性为99%。
A hemolysin gene from six streptcoccus suis type 2 strains isolated in Jiangsu was amplified by PCR using a pair of specific primers. The PCR product from strain HA9801 was sequenced and nucleotide sequence analysis revealed the hemolysin gene from strain HA9801 had a high homology to strains 1933（99%）.
出处
《中国畜牧兽医》
CAS
2006年第3期59-61,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家973项目子课题(G1999011906)
关键词
猪链球菌2型
溶血素
核苷酸序列
streptcoccus suis type 2
suilysin
nucleotide sequence
