摘要
对岩藻聚糖硫酸酯寡糖.铈配合物的制备及其对胶原蛋白的水解活性进行了研究。通过酸水解得到不同分子量的岩藻聚糖硫酸酯寡糖F1(MW〉5ooo),F2(MW1000-500),F3(MW〈1000),小分子的F3与Ce(Ⅳ)的配合效果最好,且水解胶原蛋白的活性高。通过实验确定了岩藻聚糖硫酸酯寡糖F3与Ce(Ⅳ)的配合条件以及F3-Ce(Ⅳ)配合物对胶原蛋白的最佳水解条件。
The formation of fucoidan oligosaccharide-cerium(Ⅳ ) complexes and catalytic hydrolysis of collagen from fish skin by the complexes have been studied. After the degradation of fucoidan from brown algae Laminaria japonica, the oligosaccharides F1, F2 and F3, with the molecular weight of more than 5 000, from 1 000 to 5 000 and less than 1 000, have been prepared. It is more easy for fraction F3 to form the complex with cerium(Ⅳ), compared to F2 and F3. The F3 cerium(Ⅳ ) complex has much greater proteolytic activity of collagen hydrolysis than Fl-cerium( Ⅳ ) and F2-cerium(Ⅳ ) complexes. The optimum ratio of F3 to Ce( Ⅳ ) and the optimum hydrolysis conditions have also been determined.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2006年第2期273-276,共4页
Periodical of Ocean University of China
基金
新世纪优秀人才支持计划项目(NCET-04-0642)资助