饲料型四倍体刺槐花器解剖构件组培特性差异研究初报

Primary Study on The difference among Tissue Cultures of the Components fromAnatomizing Flower of Tetraploidy Robinia pseudoacacia for Fodder

饲料型四倍体刺槐的花器可以解剖为若干构件,这些构件分别有不同的特化细胞构成,因而其组织培养特性可能有所不同。通过把这些不同解剖构件接种到不同激素浓度的MS培养基上进行培养,得出了初步的趋势性结论,这些花器构件按组织培养特性可分为两组:一组包括花萼及位于其外的解剖构件,其脱分化较易,但污染率较高;另一组包括位于花萼以内的所有解剖构件,其脱分化较难,但污染率较低。

The flower of Tetraploidy Robinia pseudoacacia for fodder can be anatomized into many Components that consist of specialized ceils that may lead the Components to be different from each other in tissue culture. By inoculating the Components on the culture medium （MS） with different Concentration of hormone the following primary trends are found out： the whole Components can be divided into two groups according to their traits in culture; group 1, including all Components from outside calyx and calyx, differentiate relatively easily but have a high contamination rate; group 2, involving all Components from inside calyx, differentiate relatively difficultly but own a low contamination rate.