蜂胶涂膜对变形链球菌生长和黏附的抑制作用

The inhibitory effect of propolis varnish on the growth and adhesion of S. mutans and S. sobrinus

目的：观察蜂胶涂膜对变形链球菌生长和黏附的抑制作用。方法：采用纸片琼脂扩散法观察10、25、50g／L和100g／L蜂胶防龋涂膜对变形链球菌c型和d型的抑菌作用。以蜂胶涂膜涂布黏附板，置变形链球菌培养液中培养，观察其抗黏附效果。结果：各浓度蜂胶涂膜及基质都能够抑制细菌生长和黏附，且抑菌作用呈现明显的浓度依赖性，100g／L涂膜组的抑菌效果与1．6g／L洗必泰溶液无显著性差异（P〉0．05）。结论：25～100g／L蜂胶涂膜均可以有效抑制变形链球菌c型和d型的生长黏附。

Objective：To observe the inhibitory effect of propolis varnish on the growth and adhesion of S. mutans and S. sobrinus. Methods：Varnishes were prepared with matrix and propolis at 0,10,25,50 and 100 g/L respectively. The bacteria of S. mutans Ingbritt c and S. sobrinus 715 were cultured on the varnish at 37 ℃ for 48 h. The effects of propolis on the growth and adhesion of the bacteria were examined by agar diffusion method and adhesion test in plastic dishes. Results：Propolis inhibited the growth of the bacteria dose-dependently and inhibited the adhesion of the bateria completely at all the tested concentrations. 100 mg/ml propolis varnish showed the same bacterial inhibiting effect as 1.6 g/L chlorhexidine solution（ P 〉 0.05 ）. Propolis at 0.625 g/L was the minimal inhibitory concentiation on adhesion of the bacteria on the varnish. Condusion：Propolis varnish can inhibit the growth and adhesion of S. mutans and S. sobrinus.