摘要
目的检测构建的PcDNA3.1/sTNFRⅠ真核表达载体在小鼠体内能否有效表达目的产物,为探索利用该载体基因治疗TNFα介导的炎性疾病奠定一定的实验基础。方法将PcDNA3.1/sTNFRⅠ载体直接注入小鼠胫前肌,ELISA法检测肌肉匀浆中的sTNFRⅠ表达。结果在注射后7d与10d,PcDNA3.1/sTNFRⅠ注射组小鼠肌肉匀浆中sTNFRⅠ表达的量均显著高于PcDNA3.1注射组(P<0.01),注射后第10天sTNFRⅠ表达量高于第7天sTNFRⅠ表达量(P<0.01)。结论采用直接注射法将PcDNA3.1/sTNFRⅠ载体转移入肌肉内可以使目的基因得到一定的表达。
Objective: To explore whether the recombinant plasmid PcDNA3. 1/ sTNFR Ⅰcould be expressed properly in mice. Methods:The recombinant plasmid PcDNA3.1/sTNFR Ⅰ and the empty vector PcDNA3.1 were directly injected into the fight tibialis anterior muscle of each 6 mice respectively. The expression product, sTNFRⅠ , in muscle homogenates was measured by ELISA 7 and 10 days after injection respectively. Results:7 days after injection sTNFR Ⅰ (mg/g) in muscle homogenates in PcDNA3.1/sTNFR Ⅰ and PcDNA3.1 injected mice was 161. 123 ±4.0 and 69.34 ±1. 489( P 〈0.01 ), 10 days after injection 191. 727 ± 3. 361 and 77. 664 ± 2. 927( P 〈0.01 ) ,respectively. In each group 10 days after injection sTNFR Ⅰ level was higher than 7 days after injection (P 〈0.01 ). Conclusion:Recombinant plasmid PcDNA3.1/sTNFR Ⅰ can be expressed in muscle of mice.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期221-223,共3页
Journal of Practical Stomatology
基金
广东省卫生厅基金资助项目(2004A119)
关键词
Ⅰ型人可溶性肿瘤坏死因子受体
重组质粒
基因表达
Soluble human tumor necrosis factor receptor Ⅰ
Recombinant plasmid
Gene expression