摘要
目的:生物信息学分析与脑缺氧密切相关的新蛋白质YZ-2,以及制备其多克隆抗体。方法:根据生物信息学分析预测出YZ-2蛋白质的二级结构、亲水性、抗原性等理化特性,以这些分析预测为依据,经过同源性检索后设计出一段由22个氨基酸组成的多肽,免疫家兔得到多克隆抗体,并用ELISA、Westernblot等方法鉴定其特异性和滴度。结果:①成功分析预测其抗原性和亲水性;②制备了抗YZ-2蛋白多克隆抗体;③以此多克隆抗体经酶联免疫吸附测定、蛋白质印迹,检测YZ-2蛋白的表达,证实此抗体效价较高、特异性强。结论:利用生物信息学较好地预测出YZ-2的亲水性和抗原性,并成功制备了其较高特异性的多克隆抗体。
AIM: To analyze YZ-2, a novel protein associated with cerebral ischemia and prepares its polyclonal antibody. METHODS: According to the bioinformatics analysis and prediction of the possible high structure, hydrophilicity and antigenicity of YZ-2, a 22-amino acid residue partial peptide of YZ-2 was synthesized. The synthesized peptide was then used to immunize. And the properties of antiYZ2 were analyzed by ELISA and Western blot. RESULTS: The hydrophilicity and antigenicity were predicted by methods of bioinformatics. The polyclonal antibody of YZ-2 was successfully obtained and its specificity and sensitivity were conformed by ELISA and Western blot. CONCLUSION: By the bioinformatics analysis and prediction, the hydrophilicity and antigenicity of YZ2 were analyzed. The antibody of YZ- 2 was successfully obtained.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第2期205-207,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(No.30472010
30572177)