强次声波对豚鼠Corti器超微结构的损伤

Ultrastructural damage of the organ of Corti induced by intense infrasound in guinea pigs

目的观察强次声波对豚鼠Corti器超微结构的损伤情况。方法将豚鼠置于频率8 Hz、强度为135 dB SPL的次声声场中连续暴露90 min。应用扫描电镜分别观测强次声波暴露后即刻(2 h内)、2天和7天时动物 Corti器超微结构的变化,计算各组耳蜗的受损率,与对照组进行比较。结果扫描电镜下见各实验组动物Corti器感觉毛细胞纤毛缺失、散乱、倒伏,表皮板等结构均有不同程度的损伤。在存活较长时期后,还可见到纤毛融合,部分细胞的表皮板破裂,以及支持细胞分离,细胞溶解,形成空洞。耳蜗受损率分别为70％-80％。结论强次声波可引起豚鼠Corti器超微结构不同程度的损伤。

Objective To study the ultrastructural changes of the organ of Corti induced by infrasound in guinea pigs. Methods Twenty albino guinea pigs were randomly divided into four groups. Fifteen guinea pigs were exposed to 8 Hz infrasound of 135 dB SPL for 90 minutes and sacrificed at 2 hours, 2 days and 7 days, respectively, after infrasound exposure. The remaining five animals serw：d as a control group. Results The ultrastructure of the inner ear was damaged to different extent. The number of outer hair cells （OHCs） of the organ of Corti decreased significantly in all experimental groups. Focal or scattered OHC losses and / or disorganized stereocilia of OHCs were found from the basal to third cochlear turns, compared with the control group. Conclusion High-level infrasound can cause damage to the inner ear of guinea pigs.