隐匿型乙肝病毒在隐源性肝病中的感染率研究

The study on the infection rate of the occult infection of hepatitis B virus in cryptogenic liver disease.

目的探讨乙肝隐匿型感染(occult infection of HBV)在隐源性肝病中的感染率。方法采用PCR技术从健康献血人员与HBsAg阴性的隐源性肝病患者(包括肝硬化、肝癌、丙肝与病因不清肝炎)血清中检测HBV X DNA,统计并分析HBV在各组中的感染率。结果隐匿型HBV在健康人群中的感染率为5%,而在隐源性肝硬化、肝癌、丙肝与病因不清肝炎中的感染率分别为30%、20%、32%、7.4%。与健康人群相比,隐源性肝硬化、肝癌与丙肝患者的HBV感染率显著增加、但病因不清肝炎患者HBV感染率却无显著差异。研究还发现,肝硬化与肝癌患者的性别/HBV抗体的检出与HBV感染率的关系不大,但丙肝患者其HBV抗体的检出与HBV感染率有密切的联系。结论本研究小范围内统计了隐匿型HBV在隐源性肝病中的感染率,并证实HBV隐匿型感染在隐源性肝硬化、肝癌患者中与性别、HBV抗体检出没有关系,而在丙肝患者中其与乙肝血清抗体的检出相关。

Objective To investigate the infection rate of the occult infection of hepatitis B virus （HBV） in cryptogenic liver disease. Methods HBV nucleic acids were extracted from sera of health blood donor and the HBV surface antigen （HBsAg） negative patients including cryptogenic cirrhosis, liver cancer, hepatitis C and cryptogenic chronic hepatitis. The HBV X DNA was tested by PCR method . Results The infection rates in health blood donor, cryptogenic cirrhosis, liver cancer, hepatitis C and cryptogenic chronic hepatitis were 5% ,30%, 20% , 32%, and 7.4%, respectively. As compared with CON （ the infection rates of health blood donor） , the infection rates of cryptogenic cirrhosis, liver cancer, and hepatitis C significantly increased, but there was no significant difference in the rate between CON and cryptogenic chronic hepatitis. There was no relationship between HBV infection rate and patients sex/the detection of HBV antibody in cryptogenic cirrhosis and liver cancer. However, the detection of HBV antibody in hepatitis C patients may be related to the occult infection of HBV. Conclusion Patient＇s sex and the detection of HBV antibody in cryptogenic cirrhosis and cryptogenic liver cancer groups may be not related to the occult infection of HBV , but the detection of HBV antibody in hepatitis C patient group may be related to the occult infection of HBV.