HPV感染与非小细胞肺癌的相关性

A study on the relationship between HPV infection and non-small-cell lung cancer

背景与目的人乳头瘤病毒(HPV)是宫颈癌的主要病因,但近来发现在非小细胞肺癌(NSCLC)中能检测到HPV,两者间的关系已经引起了人们的重视。本研究探讨人乳头瘤病毒(HPV)16、18型感染与非小细胞肺癌(NSCLC)是否存在相关性及其意义。方法应用PCR、免疫组化和TUNEL分别检测76例NSCLC及13例肺良性病变中HPV16、18型DNA及其原癌蛋白E6E7、端粒酶hTERT、P53、MDM2、桩蛋白(paxillin)和细胞凋亡的表达。结果NSCLC中HPV16、18型总阳性率为40.8%(31/76),而肺良性病变组阳性率为7.7%(1/13),差异有显著性(P<0.05)。NSCLC中HPV阳性组与阴性组之间细胞凋亡、P53、MDM2、paxillin的表达均有显著性差异(P<0.05),而端粒酶hTERT无显著性差异。HPV感染与组织学类型及淋巴结转移无关,而与组织分化程度有显著性差异(P<0.05)。结论HPV16、18型感染在NSCLC发生中可能有病因学意义,其致癌机制可能与细胞凋亡、P53、MDM2及paxillin表达变化有关。

Background and purpose： Human papillomavirus （HPV） is the main factor for cancer of the cervix, however HPV can be detected in non-small-cell lung cancer （NSCLC） recently. The relationship between them is notable. We investigated whether there was a significant relationship between HPV16, 18 infection and non-small-cell lung cancer. Methotis： PCR, immunohistochemistry and TUNEL were applied to detect HPV16, 18 DNA and oncoprotein E6E7, paxillin, telomerase hTERT, P53, MDM2 and cell apeptosis expression in 76 NSCLC and 13 benign lung specimens, respectively. Results： The positive rates of HPV16, 18 DNA in NSCLC and lenign groups were 40.8% （31/76） and 7.7% （1/13）, respectively. There was no relationship between HPV infection and clinicopathological factors of NSCLC including histoelassification and lymph node metastasis except for degree of differentiation （P 〈 0.05）. There was statistical significance with regard to cell apeptosis, P53, MDM2 and paxillin expression between HPV positive and negative group in NSCLC except telomerase hTERT （P 〈 0.05）. Conclusions： HPV16, 18 infection might have etiologic significance for NSCLC, the expression changes of cell apeptosis, P53, MDM2 and paxillin might be one of the carcinogenesis mechanisms.