survivin mRNA表达水平与高三尖杉酯碱诱导恶性血液病细胞凋亡的相关性研究

Expression of survivin mRNA in HHT-induced cell apoptosis of hematological malignancy cell lines

目的:研究surv iv in mRNA在细胞株MUTZ-1、K 562、Jurkat、RPM I、HL 60的表达水平与高三尖杉酯碱(HHT)诱导的凋亡率的关系。方法:应用流式细胞仪技术,研究HHT诱导人骨髓增生异常综合征细胞株MUTZ-1、人急性红白血病细胞株K 562、人淋巴瘤细胞株Jurkat和人骨髓瘤细胞株RPM I,以及人急性粒细胞白血病细胞株HL 60细胞凋亡。用半定量逆转录酶-多聚酶链式反应,检测上述细胞株及HHT作用MUTZ-1细胞后凋亡蛋白抑制因子surv iv in、X IAP的表达。结果:细胞株MUTZ-1、K 562、Jurkat、RPM I、HL 60均表达surv iv in、X IAP mRNA,HHT能诱导各细胞株凋亡,HHT诱导的凋亡率与细胞株surv iv in mRNA表达呈负相关。结论:surv iv in在细胞中的表达水平可能直接影响细胞对化疗药物的敏感性,可作为预测细胞耐药的指标之一,同时也可能将成为肿瘤治疗的新靶向。

Objective： To investigate the expression of survivin mRNA in hematological malignancy cells and its correlation with HHT-induced cell apoptosis. Methods： Hematological malignancy cell lines MUTZ-1, K562, Jurkat, RPMI and HL60 were treated with HHT in vitro. Cell apoptosis was observed by flow cytometry （FCM） and the expression of surviving and XIAP mRNA was evaluated with semi-quantitative RT-PCR. Results： The expression of survivin mRNA on cell lines was negatively correlated to HHT-induced cell apoptotic rate （r=-0.980, P=0.003）. There were no significant differences in XIAP expression among these 5 cell lines. Conclusion： Survivin could be used as a new marker for drug-sensitivity and a new target for treatment of hematological malignancies.