瘢痕疙瘩的差异表达基因谱研究

Differential gene expression profile of keloids: a study with cDNA microarray

目的应用基因芯片技术筛选瘢痕疙瘩组织的差异表达基因,探讨异常瘢痕发生的分子机制。方法应用含有8064个人类靶基因的表达谱芯片,检测瘢痕疙瘩和正常皮肤组织中基因的差异表达变化,筛选出差异表达基因,应用RT-PCR验证芯片结果;建立与瘢痕疙瘩形成相关的基因表达谱并进行生物信息学分析。结果与正常皮肤组织相比,瘢痕疙瘩组织中发生显著性差异表达变化的基因有277个,其中上调163个、下调114个,涉及到各种信号传递及基因调控的改变。RT-PCR检测结果与芯片结果呈一致性趋势。结论创面过度愈合形成瘢痕疙瘩是一个复杂的病理生理过程,受多种基因表达调控;差异表达基因谱的建立和研究较全面反映了瘢痕疙瘩发生的分子生物学概貌,有助于认识异常瘢痕形成的机制。

Objective To investigate the differentially expressed genes in keloids in comparison with normal skin using cDNA microarray. Methods The cDNA microarray consisting of 8064 clones of human genes was employed to detect and screen the differentially expressed genes in keloid and normal skin tissues. Semi-quantitative RT-PCR was applied to verify the results of gene microarray. Results Totally 277 differentially expressed genes were identified in keloids in comparison with normal skin tissue, including 163 up-regulated genes and 114 down-regulated ones according to the designed data filter criteria. These dif- ferentially expressed genes belonged to 26 different functional gene families involving different biological processes. RT-PCR ~ yielded results were consistent with those of microarray study. Conclusion A variety of genes are involved in the formation of keloids. The 277 differentially expressed genes comprise the differential gene expression profile ofkeloids and describe the general changes in the gene expressions in keloid at transcriptional level. Further analysis of the identified genes might help reveal the molecular mechanism of abnormal scarring.