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S-生物丙烯菊酯对人淋巴细胞的影响

Effect of S-bioallethrin on human lymphocyte
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摘要 目的运用基因芯片技术研究S-生物丙烯菊酯对正常人淋巴细胞的作用。方法取正常人外周血淋巴细胞,加入S-生物丙烯菊酯刺激,分别进行光镜、流式细胞仪、电镜、DNA梯度和基因芯片检测,研究正常淋巴细胞在S-生物丙烯菊酯刺激后的变化。结果正常淋巴细胞在S-生物丙烯菊酯刺激后发生凋亡,并有346个基因表达发生改变。结论S-生物丙烯菊酯可导致正常淋巴细胞凋亡和基因表达谱改变。 Objective To study the effect of the S-bioallethrin on human lymphocytes by microarray technique. Methods The changes of normal human lymphocytes treated with S-bioallethrin were examined with light microscope, flow cytometry, electron microscope, DNA ladder and microarray techniques. Results Morphological study showed that the lymphocytes underwent apoptosis aider S-bioallethrin exposure, which as further confirmed by the expression changes of 346 genes. Conclusion S-bioallethrin can induce apoptosis of normal human lymphocytes and changes in their gene expression profiles.
作者 刘玥 梁丽燕 马文丽 郑文岭
机构地区 南方医科大学基因工程研究所 广东省职业病防治院
出处 《南方医科大学学报》 CAS CSCD 北大核心 2006年第3期321-324,327,共5页 Journal of Southern Medical University
基金 广州市重大科技攻关项目(01-Z-005-1)~~
关键词 S-生物丙烯菊酯 淋巴细胞 凋亡 基因表达谱 S-bioallethrini lymphocyte apoptosis gene expression profiling
分类号 R114 [医药卫生—卫生毒理学]
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参考文献15

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南方医科大学学报
2006年 第3期

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