血卟啉-赫赛汀光敏免疫偶联物的制备及光敏免疫疗法研究

Preparation of hematoporphyrin-herceptin photoimmunogonjucate for photoimmunotherapy

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摘要目的制备光敏剂血卟啉(HP)-单克隆抗体赫赛汀的光敏免疫偶联物,并观察偶联物对BT-474人乳腺癌细胞的杀伤及凋亡作用。方法以碳二亚胺为缩合剂,化学制备HP-赫赛汀光敏免疫偶联物。给予波长630 nm激光照射后,用MTT法、FCM检测比较免疫偶联物和游离HP对BT-474人乳腺癌细胞的杀伤及凋亡作用。结果含同剂量HP的偶联物较游离HP对BT-474细胞的杀伤及凋亡效果明显增强(P<0.05)。结论成功制备HP-赫赛汀光敏免疫偶联物,该偶联物对BT-474人乳腺癌细胞的杀伤作用强于游离HP。 Objective To prepare photoimmunoconjugate of hematoporphyrin （HP） and herceptin, and study its killing and apoptosis-inducing effect on tumor cells BT-474. Methods HP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator. After exposure of the cells to 630 nm laser, the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry. Results Compared with free HP at equivalent dose, the immune reactivity, killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced （P〈0.05）. Conclusion The killing effect of HP-herceptin immunoconjugate is stronger than free HP on BT-474 cells.

作者陈璐罗荣城李黎波严晓丁雪梅

机构地区南方医科大学南方医院肿瘤中心

出处《南方医科大学学报》 CAS CSCD 北大核心 2006年第3期355-357,共3页 Journal of Southern Medical University

关键词光免疫疗法血卟啉赫赛汀光敏免疫偶联物 BT-474 乳腺肿瘤 photoimmunotherapy hematoporphyrin herceptin photoimmunoconjucate BT-474 cells breast neoplasms

分类号 R943 [医药卫生—药剂学] R73-36 [医药卫生—肿瘤]

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南方医科大学学报

2006年第3期

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血卟啉-赫赛汀光敏免疫偶联物的制备及光敏免疫疗法研究

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