下肢慢性静脉功能不全患者红细胞CD35和粒细胞CD11b的表达

Evaluation of the expression of CD35 on red blood cells and CD11b on neutrophils in patients with chronic venous insufficiency

目的探讨下肢慢性静脉功能不全(CVI)患者外周血红细胞CD35和中性粒细胞 CD11b表达的意义。方法采用全血流式细胞技术分析42例患者和8例正常对照者在临床、病因、解剖和病理生理(CEAP)不同临床分级中红细胞CD35和中性粒细胞CD11b的表达。结果随着 CVI临床表现的加重,C5-C6级患者CD11b表达与C2-C3级、C4级和正常对照组间差异有统计学意义(F=5．435,P<0．05);C2-C3级患者红细胞CD35表达与正常对照组间差异无统计学意义(F= 2．506,P>0．05);C4级患者红细胞CD35表达与对照组、C5-C6级患者间差异有统计学意义(P< 0．05)。结论中性粒细胞CD11b和红细胞CD35可能参与了CVI的发病过程,并在一定程度上反映病变的程度。

Objective To investigate the expression of CD35 on red blood cells （RBC） and CD11b on neutrophils in patients with chronic venous insufficiency （CVI）. Methods Anticoagulated peripheral venous blood was assayed by flow cytometry for CD35 expression on RBC and CD116 on neutrophils from 42 CVI patients in different clinical picture, etiology, anatomic distribution, pathophysiology （CEAP） and clinical classes, results were compared with that of eight normal control subjects. Result Circulating neutrophils were more reactive to CDllb in CVI C5-C6 patients. Higher expression of CD35 on RBC were observed in CA patients. Conclusion CD35 on RBC and CDllb on neutrophils may participate in the pathogenesis and to an extent reflect the severeness of CVI.