摘要
目的:观察积雪草提取物对大鼠肾小球系膜细胞(MC)增殖和钙离子的影响。方法:将血管紧张素Ⅱ(AngⅡ)作用于肾小球系膜细胞,加入不同浓度的积雪草提取物,采用MTT法和Fura-2/AM荧光指示剂负载测定MC增殖和细胞内[Ca2+]I的变化。结果:MC一经AngⅡ刺激后增殖显著,并且Ca2+浓度明显升高,而积雪草提取物对AngⅡ刺激下的MC增殖和Ca2+水平有抑制和降低作用,并呈一定的剂量依赖性。结论:积雪草提取物可能通过降低MC内Ca2+的水平,从而达到抑制MC增殖的目的。
Abstract：Centella asiatica（ASA） on proliferaton and intracellular free calcium of rat glomerular mesangial cells（MC）. Methods： Rat MC were incubated with angiotensin Ⅱ （Ang Ⅱ）, To observe the proliferation and intracellular free calcium concentration by the MTT method and flurescence measurement with Fura-2/AM after given the extracts from ASA. Results after stimulation of Angiotensin Ⅱ, the proliferation and intracellular free calcium concentration raised obviously, and ASA could dose-dependently inhibit the the proliferation and intracellular free calcium concentration. Conclusion ASA could inhibit MC proliferation by decreasing the intracellular free calcium concentration.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2006年第1期22-23,共2页
JOURNAL OF BASIC CHINESE MEDICINE
关键词
积雪草
肾小球系膜细胞
游离钙
Centella asiatica
mesangial cells
free calcium
