HPLC法测定延胡索药材中季胺型生物碱的含量

HPLC Determination of Quaternary Alkaloid from Herba Corydalis Tuber

目的：建立测定延胡索药材中季胺型生物碱含量的HPLC方法.方法：色谱柱为迪马公司钻石牌C18柱（250mm×460mm）;流动相为0.6%冰醋酸水溶液（合0.06%三乙胺）-乙睛（77：23）;检测波长：348nm;流速：1.0ml/min.结果：在此色谱条件下延胡索药材中季胺型生物碱小檗碱可以得到很好的分离,盐酸小檗碱在0.02～0.1μg间呈线性关系（r=0.9998）.结论：本方法重现性好,灵敏度高,结果准确可靠.

Objective： To develop a method for assay of Quaternary Alkaloid from herba corydalis tuber Methods： Dikma DiamonsilTM ODS （ 250mm × 460mm） column was applied, and mobile phase was 0.6% acetic acid （which contain 0.06% of triethylamine）-acetonitrile （77：23）. The UV detection wavelength was 348nm,the flow rate was 1.0ml· min^-1, Results： The selected components were successfully separated under this chromatographic system, the liner range was 0.02 - 0. 1μg（ r = 0.9998） for hydrochloride Palmatine. Conclusion：The method is reliable, sensitive and accurate for improve the certification of herba corydalis tuber.