摘要
记述8种当前用于害虫抗药性检测的分子生物学方法:专一性等位基因PCR扩增;PCR-单链构象多态性;固相微测序反应;限制性片段长度多态性;PCR-寡核苷酸探针斑点杂交;随机扩增多态性DNA;Northern斑点杂交;固定化人工膜-高效液相色谱。评述各种检测技术基本原理、在害虫抗性检测中的优缺点及应用范围。
Eight molecule-based techniques for detection of resistance in pests were described, which included PCR Amplification of Specific Alleles （PASA）, PCR-single-strand conformation polymorphism （SSCP）, Restriction Fragment Length Polymorphism （RFLP）, Solid-Phase Minisequencing （SPM）, PCR-allele specific oligo nucleotide probe （PCR-ASO）, Randomly Amplified Polymorphic DNAs （RAPD）, Northern blotting and Immobilized artificial membrane high performance liquid chromatography （IAM-HPLC）. It revealed the basic elements, advantages or disadvantages and application scopes of these detecting techniques responsible for insecticide resistance in insects.
出处
《寄生虫与医学昆虫学报》
CAS
2006年第1期57-63,共7页
Acta Parasitologica et Medica Entomologica Sinica
关键词
害虫
抗药性
分子检测
Pest
Resistance
Molecular
Detection
