含CpG基序寡核苷酸对rHBsAg免疫小鼠脾脏组织及细胞增殖的影响

Influence of CpG ODN on Histological Change and Lymphocyte Proliferation of Murine Spleen Immunized with rHBsAg

目的探讨合成含CpG基序的寡核苷酸(CpGODN)与重组乙型肝炎表面抗原(rHBsAg)联合免疫对小鼠脾脏淋巴组织及细胞增殖的影响。方法经后腿胫骨前肌初次免疫BALB/c小鼠,2周后加强免疫1次;常规石蜡切片,H-E染色,观察脾脏组织学改变;3H-TdR掺入法检测免疫小鼠脾脏淋巴细胞增殖反应。结果抗原+CpG组脾脏可见明显的组织学改变。加CpG与不加CpG的2组比较,淋巴细胞增殖反应显著增强。结论CpGODN能够有效刺激小鼠脾脏组织T细胞区淋巴细胞增生,生发中心明显增大,而且能够显著增强淋巴细胞增殖反应。

Objective To explore the influence of combined immunization with the synthetic oligonuclentide containing CpG motif（ CpG ODN） and rHBsAg on the histological change and lymphocyte proliferation of murine spleen. Methods Twenty-five BALB/c mice were randomly divided into four test groups and one control group, five for each,and immunized twice with rHBsAg,HB vaecine,rHBsAg ＋ CpG ODN, HB vaccine ＋ CpG ODN and physiological saline respectively, at an interval of 2 weeks. Prepare the mufine spleens into routine paraffin sections for observation of histological change by H-E staining. Determine the proliferation of lymphocytes by ^3H-TdR incorporation method. Results Significant histological change was observed in the murine spleens in rHBsAg ＋ CpG ODN group. Compared with those of mice in rHBsAg and HB vaccine groups ,the lymphocyte proliferations of mice in rHBsAg ＋ CpG ODN and HB vaccine ＋ CpG ODN groups were significantly enhanced. However,compared with those of mice in control group,the lymphocyte proliferations of mice in all the four test groups were significandy enhanced. Conclusion CpG ODN caused the histological change of murine spleen, such as extension of germinal center, and enhanced the lymphocyte proliferation signifieandy. It indicated that CpG ODN might be a potential Thl type adjuvant of HB vaccine.