呋喃唑酮促睾丸细胞凋亡作用

Induction of Apoptosis of Testicular Cells by Nitrofurazolidone(NFZ)

目的探讨呋喃唑酮(NFZ)诱导大鼠睾丸细胞凋亡的作用,为指导临床药物及禽畜药物的使用提供一定的理论依据。方法采用免疫组化、细胞培养、亚细胞器分离、无细胞体系及免疫沉淀技术,观察睾丸细胞在NFZ作用下形态变化;检查睾丸细胞及其无细胞系统在NFZ作用后的DNA梯状电泳图谱以及与凋亡相关的Bcl-2、caspase-3等因子表达。结果睾丸细胞在NFZ作用下,形态变化具有明显的凋亡特征。DNA电泳出现梯状电泳带,即DNA片段化。分析与凋亡相关的因子表达,均符合细胞程序死亡的规律。结论呋喃唑酮可促进精子细胞凋亡。

Objective To explore the molecular mechanism of induction of testicular cell apoptosis by nitrofurazolidone（NFZ） and provide theoretical basis for guiding the application of drugs for human and animals. Methods Observe the morphological change of testicu/ar cells under induction of NFZ by immunohistochemical method, cell culture, separation of subcellu/ar organellcs, cell-free system and immuno-precipitation. Examine the DNA electrophoretic profile of testicular cells after induction with NFZ as well as the expressions of Bcl-2 and caspase-3, using cell-free system as control. Results The morphological change of testicular cells induced with NFZ showed the characteristic of apoptosis. Scalafiform bands were shown on the DNA electrophoretic profile of the induced cells, which indicated the fragmentation of DNA. The expressions of Bcl-2 and caspase-3 met the regulation of apoptosis. Conclusion NFZ induced the apoptosis of testicular cells.