有氧运动对大鼠动脉粥样硬化发生发展过程中心肌CGRP、AngⅡ的影响

Influence of Aerobic Exercise on CGRP and AngⅡin Myocardium in the Process of Atherosclerosis Evolution

通过探讨不同负荷有氧运动对大鼠动脉粥样硬化发生发展过程中心肌CGRP、AngⅡ含量的影响,为制定动脉粥样硬化运动处方提供一定的理论参考。运用雄性Wistar大鼠40只,随机分为正常对照组(NC,n=10);动脉粥样硬化组(AS,n=10);动脉粥样硬化+60 min有氧运动组(ASAT1,n=10);动脉粥样硬化+120min有氧运动组(ASAT2,n=10)。建立大鼠动脉粥样硬化和有氧运动模型。放免法测定心肌中CGRP和AngⅡ含量。结果发现:AS组与NC组相比,心肌CGRP显著降低(P<0.05),AngⅡ显著升高(P<0.05)。ASAT1、ASAT2两组与AS组相比,心肌CGRP有升高趋势,ASAT1组AngⅡ显著低于AS组(P<0.05)。ASAT2与ASAT1相比,心肌AngⅡ显著升高。表明:1)60 min有氧训练可以使大鼠动脉粥样硬化发生发展过程中心肌组织CGRP升高,AngⅡ降低,延缓动脉粥样硬化的进程。2)120 min有氧训练与60 min有氧训练相比,大鼠心肌CGRP降低,AngⅡ升高。从某种程度说明较长时间有氧运动并不能进一步改善动脉粥样硬化。

In order to probe into the mechanism of exercise preventing atheroselerosis , calcitonin gene-related peptide （CGRP） and angiotensin Ⅱ （ Ang Ⅱ ） in myocardium were observed under different aerobic exercise workload in the process of atheroselerosis evolution. 40 male Wistar rats were divided into four groups at random： 1 ）normal control group （NC, n =10）; 2） atheroselerosis group （AS, n = 10）; 3） atheroselerosis and aerobic exercise for sixty minutes everyday （A SAT1, n = 10）; 4） atherosclerosis and aerobic exercise for one hundred and twenty minutes everyday （ASAT2, n = 10）. Atheroselerosis and aerobic exercise models were set up and radioimmunoassay was used to detect the levels of CGRP and Ang Ⅱ in myocardium. The results indicated that when compared with NC group, CGRP appeared significant declining tendency but Ang Ⅱ indicated significant ascending tendency in myocardium （ P 〈 0.05）. When compared with AS group, both CGRP and Ang Ⅱ showed ascending tendency, but significant difference appeared only in ASAT1. Compared with ASAT1, Ang Ⅱ in myocardium showed significant ascending tendency. Several conclusions can be summarized as follows： 1） Sixty minutes aerobic exercise helped to suppress excessive hyperplasia of the smooth muscle cell of blood vessel and delay the process of the atheroselerosis by increasing CGRP but decreasing AngⅡ in myocardium. 2） Compared with sixty minutes aerobic exercise, one hundred and twenty minutes aerobic exercise decreased CGRP but increase AngⅡ in myocardium. Taking into account the levels of lipoprotein and changes of aorta under microscope, conclusion may be drown that too much minute of arobic exercise did not do better for atheroselerosis than short time of arobic exercise did.