免疫组化法检测戊型肝炎肝内病毒抗原

DETECTION OF HEJPATITIS E VIRUS ANTIGEN IN HEPATIC TISSUS BY IMMUNOHISTOCHEMICAL METHOD

用免疫组化方法检测肝组织内抗原为病原诊断重要手段之一。本文用此法检测急性戊型肝炎（戊肝）肝活检组织１２例、病后２８个月临床、肝功能和肝组织均未恢复正常的１５例标本，检测阳性结果分别为１００％和７３．３％。另有一例由于母亲患重症戊肝而早产的胎儿肝组织内也显示阳性。本结果除对病原诊断有重要意义外，对戊肝的发病机理，慢性化和宫内感染提出新论点。

mmunohistochemical method is an important technique for detection of hepatitis E virusantigen(HEV-Ag)in hepatic tissue. The biopsied liver specimens of 12 cases with acutehepatitis E and of 15 unrestored cases followed up for 28 months were examined,and theHEV-Ag positive rates were 100%and 73.3%,respectively. In addition,the liver specimenof a premature infant,whose mother was seriously ill with hepatitis E, was detected posi-tive. These results may be of signifigance to the pathogenic diagnosis. The authors ofered afresh point of view on pathogenesis,chronicity and intrauterine infection.