大鼠骨髓细胞悬液培养及中药953对该培养中CFU－F形成影响的研究

Study on culture of rat bone marrow cell suspension and the effect of the traditional Chinese medicine 953 on this culture

Ｗｉｓｔａｒ大鼠分成３组：高剂量组、低剂量组、对照组进行中药９５３喂养。８０ｄ后，杀鼠，取股骨制成骨髓细胞悬液，于微孔滤膜上培养１２ｄ，观察ＣＦＵ－Ｆ形成情况。发现随着培养时间的延长，骨髓基质细胞形成集落增多，在基质细胞形成的集落周围有少量淋巴细胞，而无基质细胞的空白区，则无淋巴细胞。说明骨髓内存在的基质祖细胞，在体外培养中，可形成集落，并支持、调节造血细胞的增殖分化，同时，由实验所得数据知，高剂量组、低剂量组分别与正常组比较，经／检验，Ｐ＞０．５０，差别不显著，说明该中药虽然对骨髓ＣＦＵ－Ｆ形成有影响，但影响不大。

Wistar rats were divided into three groups:(1)high dosage group,(2)low dosagegroup,and(3)control group. After feeding them with traditional Chinese medicine for 80 days,wekilled the rats and removed thigh-bone,then cell suspension was prepared frorn the thigh-bone mar-row. We cultured the suspension for 12 days on the millipore filter membrane,and observed the forma-tion of CFU-F. We found that colony of bone marrow stromal cell was increasing with the time ex-tending; there was a little lymphocytes around the colony, whereas there was no lymphocyte in theclear area without stromal cell.This showed that there was CFU-F(Fibroblastoid colony formingcells)in bone marrow,CFU-F could form colony and support,adjust multiplication and differentia-tion of blood forming cells in vitro. Moreover,according to the data acquired from this experiment,wefound that the traditional Chinese medicine 953 could affect the formation of the colony of bone mar-row stromal cell extraordinarily.