不同来源的副溶血性弧菌生物学特性研究

Biologic Characteristics of Vibrio parahaemolyticus from Different Sources

为掌握浙江省不同来源的副溶血性弧菌的血清型分布及毒力基因携带情况,采用血清凝集试验对333株副溶血性弧菌进行血清型分型,采用PCR技术检测副溶血性弧菌的毒力基因TDH。临床副溶血性弧菌菌株血清型分布于7个O抗原群,以O3为主,占临床菌株数的71.81%;O4血清型与往年相比有所上升,占到了16.60%。海产品菌株的血清型分布于8个O抗原群,以O5为主,占所有海产品菌株的47.30%。临床菌株的TDH检出率为95.65%,海产品菌株的TDH检出率为6.06%。血清型相同但来源不同的菌株的TDH检出率不同。浙江省从海产品中分离出的副溶血性弧菌菌株与分离自食源性疾病患者的副溶血性弧菌菌株的主要血清型、毒力基因TDH都存在差别。该工作为预防副溶血性弧菌引起的食品中毒提供了科学依据。

To understand the serotype and the virulent genotype of the Vibrio parahaemolyticus isolated from different sources in Zhejiang Province, the serotype was tested by serum agglutination; PCR technology was applied to measure the TDH （thermostable directs hemolysin） virulence gene. It was found that the serotypes of Vibrio parahaemolyticus isolated from clinical patients were distributed in 7 O-antigen groups. Among them, 03 was the dominating type with 71.81 % prevalence in the isolations from clinical patients, and the proportion of O4 type ruse to some extent accounting for 16.60% compared with last year. The serotype in strains isolated from seafoods distributed in 8 O-antigen groups, in which the O5 type prevailed （47.30%）. It was demonstrated that the virulence gene TDH could be detected to be 95.65% in clinical isolations employing the PCR technology. In contrast, there was only 6.06% TDH positive rate in strains isolated from seafoods. Moreover, the TDH positive rate varied with the source of the bacteria despite of sharing the same serotype. There was difference in serotype and genotype of TDH between Vibrio parahaemolyticus strains isolated from seafoods and from patients suffering from foodborn disease.