Hygicult载片培养法快速检测表面大肠菌群的效果评价

Evaluation of Hygicult CF Dipslides Method in Rapid Examination of Coliform on Surfaces in Food Production Areas

为探讨Hygicult载片培养法快速检测食品生产加工单位和餐饮单位环节表面大肠菌群的效果,对代表3种不同清洁程度的保健品生产单位、裱花蛋糕生产单位和餐饮单位的636件环节样品,以及实验室人工污染标准大肠杆菌的样品分别用载片培养法和常规发酵法进行检测,比较两种检测方法的符合率。在现场样品中,两种检测方法的总体符合率为97.6%,其中,按食品行业分类,保健品生产单位、裱花蛋糕生产单位和餐饮单位样品检测结果的符合率分别为98.0%,96.2%和93.8%;按样品种类分类:容器、工具、操作台、手、裱带、碗碟、砧板和刀具检测结果的符合率分别为100%、100%、100%、100%、96.5%、96.4%、93.2%和89.2%;按样品材质分类:不锈钢、瓷器、手、塑料、木质和布质检测结果的符合率分别为100%、100%、96.5%、95.0%、91.8%和89.2%;上述两法检测结果比较均差异无显著性(P>0.05)。在实验室人工污染样品中,两种检测结果一致。Hygicult载片培养法可以代替常规发酵法作为食品行业环节表面大肠菌群污染的快速检测方法。

To evaluate the Hygicult CF dipslides method in on-site examination of coliform on the surfaces in food production areas and catering places, the hygicult CF dipslides method and traditional zymotechnic method were used to make parallel examinations of coliform on the surfaces of health food production areas, flowered cake shops and catering places which represented three different levels of contamination. The total number of samples was 636. The two methods were also used to examine the coliform on the stainless-steel surfaces artificially contaminated with E. eoli （ATCC）. The results the samples taken from the food production areas： The overall rate of coincidence of the results from the two methods was 98.0%. According to the sampling sites, the rate of coincidence of the results from the two methods on the surfaces of examination was 98.0% in the health food production areas, 96.2% in the flowered cake shops and 93.8% in the catering places. According to the type of the samples, the rate of coincidence of the results frmn the two methods was 100% on the surfaces of the food containers, cooking utensils and the operating tables, 96.5% on the surface of the conveyer belts, 96.4% on the surface of the bowls, 93.2% on the surface of the cutting-boards and 89.2% on the surface of the cutting-knifes. According to the material of the samples, the rate of coincidence of the resuhs from the two methods was 100% on the surface of stainless steel, 96.5% on the surface of chinaware, 95.0% on the worker＇s hands, 91.8 % on the plastic surfaces and 89.2 % on the fabric surfaces. There was no statistical difference between the results from the two methods （P 〉 0.05）. The samples taken from the artificially contaminated stainless-steel surfaces： the two methods got similar results. Therefor, Hygicult CF dipslides method can replace the traditional zymotechnic method in examining the coliform on the surfaces in food production areas.