Effects of hyperbaric oxygen and Pgg-glucan on ischemic colon anastomosis

瞄准：在颜色表面的外科，吻合失败仍然是在局部缺血的一个问题。这里，我们在冒号上分析了高比重的氧和 beta-glucan 的效果吻合在是化学家状况。方法：在书籍的右页 sigmoid 区域的结肠的切除术和吻合在四组相等的数字的四十只 Wistar 白化体老鼠被做。冒号肠系膜被绑扎导致局部缺血。第一个组是控制组。第二个组的题目与高比重的氧被对待；有 glucan 的第三个组并且向前，组与两个被对待。在向前白天，老鼠被牺牲的、吻合的片断是吻合的层次衬里的 resected 和爆炸压力和羟脯氨酸被测量。结果：从控制组的第二和第三个组的爆炸压力差别是有意义的(P【0.01 ) ；向前，组与控制(P【0.001 ) 显著地不同。爆炸压力水平(P】0.05 ) 上的对待的组之间没有差别。在所有对待的组的羟脯氨酸层次显著地与控制组不同(P【0.001 ) 。羟脯氨酸层次在向前组是比第二和第三个组(P【0.001 ) 的那些高级的。在在爆炸压力和羟脯氨酸层次(P】0.05 ) 的第二和第四个组之间没有有效差量。结论：高比重的氧和 glucan 改善愈合在化学家冒号由 anti-microbic 吻合，有免疫力的刺激性质并且当一起联合了时，似乎 synergistically 行动。

AIM： In colorectal surgery, anastomotic failure is still a problem in ischemia. Here, we analyzed the effects of hyperbaric oxygen and beta-glucan on colon anastomoses in ischemic condition. METHODS： Colonic resection and anastomosis in rectosigmoid region were done in forty Wistar-Albino rats of four groups of equal number. Colon mesentery was ligated to induce ischemia. The first group was the control group. The subjects of second group were treated with hyperbaric oxygen; the third group with glucan and the forth group were treated with both. At the forth day, rats were sacrificed, anastomotic segment was resected and burst pressures and hydroxyproline levels of anastomotic line were measured. RESULTS： The burst pressure difference of second and third groups from the control group were meaningful （P〈0.01）; the forth group differed significantly from the control （P〈0.001）. There was no difference between the treated groups on burst pressure level （P〉0.05）. The hydroxyproline levels in all treated groups were different from the control group significantly （P〈0.001）. Hydroxyproline levels in the forth group were higher than those of the second and the third groups （P〈 0.001）. There were no significant differences between the second and the fourth groups in burst pressure and hydroxyproline levels （P〉 0.05）. CONCLUSION： Hyperbaric oxygen and glucan improve healing in ischemic colon anastomoses by anti-microbic,immune stimulating properties and seem to act synergistically when combined together.