摘要
大多数细胞的生物学功能都是由蛋白复合体而非单一蛋白来完成的,所以识别和分析蛋白复合体的组分是研究蛋白功能所必需的。串联亲和纯化技术(TAP)能在生理条件下有效地分离、鉴定蛋白复合体,而无需知道复合体的组成或功能,目前已成功地应用于酵母及其他生物体中的蛋白复合体的成分分析。与质谱技术联合应用可以识别与目的蛋白相互作用的蛋白,进而揭示蛋白质相互作用网络及其功能。
It＇s a multi-protein complex and not single protein that accomplish most of cellular activity. Therefore, to identify and analyze the composition of protein complex is necessary for studying the function of proteins. Tandem affinity purification technique（TAP） enables the effective purification of complex under physiological conditions without knowledge of the complex composition and function. Now, it has been adapted to analyze the composition of the protein complexes in yeast. Combined with mass spectrometry, TAP can identify the interacting proteins of target protein and offer great help for opening protein interacting network and function out.
出处
《基础医学与临床》
CSCD
北大核心
2006年第3期246-251,共6页
Basic and Clinical Medicine
