壳聚糖载体介导的骨关节炎基因治疗

Chitosan-based Gene Therapy for Experimental Osteoarthritis

目的:探讨由壳聚糖载体介导的骨性关节炎的基因治疗。方法:分别制备壳聚糖pcD-NA3.1-IL-1Ra和壳聚糖pcDNA3.1-IL-10纳米粒复合物,采用该复合物转染体外培养的正常原代兔关节软骨细胞,并将其作为药物直接注射进骨关节炎模型兔关节腔内。分别采用RT-PCR、ELISA和免疫组化方法从mRNA水平和蛋白水平检测目的基因的转染情况,并对治疗后实验动物的关节软骨和滑膜进行大体组织学观察。结果:正常原代软骨细胞转染后,在mRNA水平上检测到外源基因的水平升高;体内转染后,在蛋白水平上检测到目的蛋白在关节软骨内有阳性染色,且经基因治疗的兔关节软骨的破坏程度明显轻于未经基因治疗的对照组。结论:壳聚糖是一种较理想的基因载体,可用于早期骨性关节炎的基因治疗。

Objective To study the gene therapy for osteoarthritis by chitosan gene delivery system. Methods Chitosan - DNA nanoparticles were prepared according to the complex coacervation using the plasmid gene of pcDNA3.1 - IL - 1Ra and pcDNA3.1 - IL - 10 respectively. Each kind of the complex was transfected to primery chondrocytes and directly injected to the knee joints. Then gene expression was detected by methods of RT- PCR, ELISA and immunohistochemical staining. Results Clear expression of IL - IRa and IL - 10 were detected in the experimental knee joint synovial fluid respectively. Immunohistochemical analysis also confirmed that each of the protein was clearly detected in a few areas of the corresponding articular cartilage. A significant reduction was also noted in the severity of histologic cartilage lesions in both of the experimental groups. Conclusion Chitosan nanoparticles is compellent gene delivery system for the early osteoarthritis gene therapy.