严重急性呼吸系统综合征后骨坏死与抗心磷脂抗体

Post-SARS osteonecrosis and anticardiolipin antibodies

[目的]测定严重急性呼吸系统综合征(severe acute resp iratory syndrom e,SARS)后骨坏死患者的抗心磷脂抗体,探讨SARS后骨坏死的病因学,以便用于非创伤性骨坏死的早期诊断和易感人群的筛选。[方法]取62例SARS后骨坏死患者抽取空腹肘静脉血,另取健康人群52名为对照,应用酶联免疫吸附法测定抗心磷脂抗体的IgA、IgG和ISM,并检测血浆凝血酶原时间(PT)和凝血酶时间(TT),凝血酶原活动度(PTA)和国际标准化值(INR)4项常规出凝血检查。[结果]SARS后骨坏死患者抗心磷脂抗体阳性者多见。骨坏死组62人中有21人出现抗心磷脂抗体阳性,占33.9%,其中IgA(11.33±11.209)APL,IgG(5.127±5.927)GPL,IgM(17.821±10.606)MPL。对照组52人中仅有4人出现异常,占7.7%,其中IgA(10.702±3.126)APL,IgG(5.184±4.780)GPL,IgM(14.684±5.516)MPL,经独立样本t检验和卡方检验处理,两组之间有显著性差异(P<0.05)。血浆凝血酶原时间(PT)和凝血酶时间(TT),凝血酶原活动度(PTA)和国际标准化值(INR)4项常规出凝血检查两组之间无显著性差异。[结论]SARS后骨坏死患者的抗心磷脂抗体阳性率增多,抗心磷脂抗体可能在骨坏死发生中起作用。

[ Objective] To explore the correlation between anticardiolipin antibodies and post-SARS patients with osteonecrosis, investigate the etiology of pest-SARS osteonecrosis, and select the sensitive molecular symbols for early diagnosis and distinguish the high risk population. [ Method] The studied subjects were divided into two groups. Sixty-two pest-SARS patients with osteonecrosis was one group, and 52 matched healthy people was as normal controlled group. Empty stomach blood samples from cubital veins were collected from beth groups. IgA, IgG and IgM of anticardiolipin antibodies types were examined by ELISA. [ Result ] Twenty-one of 62 pest-SARS osteonecrosis patients were tested at least one type of anticardiolipin antibodies positive, among them, with IgA （11.33 ± 11.209） APL, IgG （5.127 ±5.927） GPL, IGM （17.821 ± 10.606） MPL Four of 52 control group persons were detected positive, with IGA （ 10.702 ± 3. 126） APL, IgG （5. 184 ±4.780） GPL, IgM （ 14. 684 ±5. 516） MPL. There were significant differences between two groups, while, there were no difference in prothrombin time （PT）, thrombin time （TF）, prothrombin activity （PTA） and international normalized ratio （INR） . [ Conclusion] The study indicates an increased positive incidence of anticardiolipin antibodies in post-SARS patients with osteonecrosis. This has reflected that anticardiolipin antibodies may play a role in osteonecrosis.