固相萃取高效液相色谱法测定姜黄中的苏丹色素

Determination of sudan dyes in curcuma by SPE-HPLC

研究了姜黄中4种苏丹色素的液相色谱测定方法。姜黄中4种苏丹色素经正己烷提取，Supelco中性氧化铝小柱净化，色谱柱为ZORBAX Eclipse XDBC18，以乙腈-水为流动相，采用梯度洗脱，二极管阵列检测器测定。该法检出限分别为0．040，0．058，0．038，0．060mg／kg，回收率在84．5％～94．3％之间，相对标准偏差（RSD）≤4．5％。

A method for the determination of sudan dyes in curcuma, including sudan Ⅰ , Ⅱ , Ⅲ, Ⅳ was studied. Sudan dyes were extracted from eurcuma with hexane, purified by Supelco SupeleleanTM LC-Alumina-N SPE column, analysed by LC on a column of ZORBAX Eclipse XDB-C18with acetonitrile-water as mobile phase, elution gradient was selected and detected at 478 and 520 nm with DAD （Diode Array Detector）. Detection limit was 0.040, 0.058, 0.038, 0.060 mg/kg respectively and the recoveries of the four dyes were 84.5 % - 94.3 %, the relative standard deviation was less than 4.5 %.