信号调节蛋白α1在胆管细胞性肝癌中的表达及生物学意义

Expression and biological significance of signal regulatory protein al in bile duct cellular hepatocarcinoma

目的观察信号调节蛋白 Sirp α1在胆管细胞性肝癌中的表达。方法选取手术切除胆管癌和癌旁组织24例,10%甲醛固定24 h 后石蜡包埋切片,采用免疫组织化学方法测定胆管癌和癌旁组织 Sirp α1表达。结果 Sirp α1在胆管癌细胞胞浆散在淡黄色表达,癌旁胆管细胞胞浆棕黄色表达,表达差异显著(P<0.05),其表达程度与胆管癌肿瘤大小、组织分化程度、有无癌栓和子灶和淋巴结转移与否有关,肿瘤越大和分化程度越差,伴有子灶、胆管和门静脉癌栓者,Sirp α1表达越低(P<0.05),而与血 AFP 和 CA_(19-9)高低无关(P>0.05)。结论 Sirp α1作为一种负向调控因子参与了胆管细胞性肝癌的发生发展,但关于其详细调控机制尚待进一步研究。

Objective To observe the （Sirp α1） in bile duct cellular hepatocarcinoma. changes in expression of signal regulatory protein α1 Methods After the bile duct carcinoma and paracarcinoma tissues were obtained from 24 patients with intrahepatic cholangiocarcinoma, fixed with 10% formaldehyde for 24 h, embedded with paraffin and sectioned, the expression of Sirp α1 was determined with immunohistochemistry. Results The light yellow staining of cytoplasm was observed in dispersed cholangiocarcinoma cells and the brown yellow staining of cytoplasm was seen in diffuse paracarcinoma cells. The expression level of Sirp α1 was significantly different between the carcinoma tissue and paracarcinoma tissue （P〈0.05）. The expression degree of Sirp α1 was significantly associated with tumor size, tissue differentiating degree, tumor embolus, satellite focus and lymph node metastasis （P〈0. 05） but not related to AFP and CA199 （P〉0.05）. Conclusions Sirp α1, as a negative regulatory factor, may be involved in occurrence and development of intrahepatic cholangiocarcinoma. However, it is necessary to further study on its regulating mechanism in detail.