摘要
一种单个分子察觉技术被联合开发作为壁炉隧道 poly (dimethylsiloxane )/glass 微能流动的薄片和荧光关联能谱法(FCS ) 。这个方法成功地被用来在包含荧光黄和玫瑰精绿色 succinimidyl 酉旨的混合决定二个模型部件的比例。
A single molecule detection technique was developed by the combination of a single channel poly (dimethylsiloxane)/glass micro-fluidic chip and fluorescence correlation spectroscopy (FCS). This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
基金
This work was financially supported by the National Natural Science Foundation of China. (No.20271033, 20335020, 90408014).
关键词
荧光关联能谱法
微射流芯片
单分子检测
玫瑰精
Fluorescence correlation spectroscopy, micro-fluidic chip, single molecule detection