摘要
应用氢过氧化枯烯作用于体外培养大鼠心肌细胞，观察其对ＤＮＡ的影响。结果表明，氢过氧化枯烯引起心肌细胞￣３Ｈ－ＴｄＲ掺入降低、ＤＮＡ断裂，脂质过氧化物含量增加。提示氢过氧化枯烯引发了心肌细胞的脂质过氧化过程，说明培养心肌细胞ＤＮＡ对自由基损伤作用是敏感的。
The effect of cumene hydroperoxide on DNA in cuItured neonatal rat heart cells wasinvestigated.The results showed that cumene hydroperoxide could cause the decrease of H-TDR incor-poration into DNA and increase of DNA strand break with the accumulation of lipid peroxide in thecells.These indicated that cumene hydroperoxide initiated and facilitated freeradical damage and lipidperoxidation in the myocyte cultures and suggested that DNA was sensitive to free·radical damage incultured cardiac cells
出处
《白求恩医科大学学报》
CSCD
1996年第3期226-228,共3页
Journal of Norman Bethune University of Medical Science
关键词
过氧化氢
DNA
心肌细胞培养
心肌损伤
hydrogen peroxide/AD
malondialdehyde/An
heart cell
cuItured
cumene hydroper-oxide
