摘要
比较了6%的DMA和11%的甘油冷冻洪山公鸡精液后精子在存活率、顶体完整率方面的差异。结果表明,用DMA做冷冻保护剂的颗粒型冷冻精液解冻后的精子存活率和顶体完整率分别为0.320±0.041和0.600±0.049,用甘油做冷冻保护剂的颗粒型冷冻精液解冻后的精子存活率和顶体完整率分别为0.313±0.036和0.597±0.040,两者差异不显著。用DMA做冷冻保护剂的细管型冷冻精液解冻后的精子存活率和顶体完整率分别为0.388±0.034和0.652±0.042,用甘油做冷冻保护剂的细管型冷冻精液解冻后的精子存活率和顶体完整率分别为0.433±0.054和0.750±0.048,前者在精子存活率(P<0.05)和顶体完整率(P<0.01)方面都显著低于后者。
In this study 6% of DMA and 11% of glycerol were used respectively as cryoprotectants to freeze Hongshan cocks＇ spermatozoa in both pellets and straws, and the cryoprotective differences in survival rates, acrosomic integrity rates of frozen-thawed spermatozoa were examined. The results showed that the survival rate and acrosomic integrity rate of spermatozoa in pellets were 0.320±0.041 and 0.6004±0.049 respectively when 6% of DMA was used as cryoprotectant, the survival rate and acrosomic integrity rate were 0.313±0.036 and 0.597±0.040 when 11% of glycerol was used to freeze cocks＇ semen in pellets. The differences between them were not significant. When 6% of DMA were used to freeze spermatozoa in straws, the survival rate and the acrosomic integrity rate were 0.388±0.034 and 0.652±0.042 respectively, the survival rate and acrosomic integrity rate were 0.433±0.044 and 0.750±0.048 respectively when 11% of glycerol was used as cryoprotectant to freeze spermatozoa in straws. Test showed that the survival rates （P〈0.05） and the acrosomic integrity rates （P〈0.01） of the former were both significantly lower than that of the latter.
出处
《湖北农业科学》
北大核心
2006年第2期220-222,共3页
Hubei Agricultural Sciences
基金
湖北省教育厅青年基金项目(2004D016)
