基质金属蛋白酶(MMP)9、MMP2 mRNA及基质金属蛋白酶抑制剂2 mRNA在人膀胱移行细胞癌中的表达及意义

Expression of MMP9, MMPE2 mRNA and TIMP2 mRNA in bladder transitional cell carcinoma and its clinical significance

目的探讨膀胱移行细胞癌中基质金属蛋白酶(MMP)9、MMP2及基质金属蛋白酶抑制剂2(TIMP2)mRNA表达与膀胱肿瘤恶性程度和侵袭性之间的关系及其临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测36例膀胱移行细胞癌及5例正常膀胱组织MMP9、 MMP2、TIMP2 mRNA的表达。结果 MMP9、MMP2 mRNA随着肿瘤分级分期的增高,表达明显增强[x-值(MMP9)G1:1．218,G2:1．681,G3:1．811;T2-T4:1．840,Tis-T1:1．399;P<0．01; (MMP2)G1:1．323,G2:1．694,G3:2．060;T2-T4:1．950,Tis-T1:1．505;P<0．01],且明显高于正常膀胱组织中的表达[x值(MMPg)0．455,(MMP2)0．461;P<0．01]。TIMP2 mRNA随肿瘤分级的增高而明显减少(x值:G1:1．489,Gn:1．391,G3:0．580;P<0．01)。结论 (1)MMP9、MMP2 mRNA的高表达与BTCC的恶性程度呈正相关;(2)TIMP2 mRNA的表达与BTCC的恶性程度呈负相关。

Objective To investigate the interrelationship between the expression of MMP2, MMP9, TIMP2 mRNA and the infiltration ability of bladder tumor, and its clinical significance. Methods Using reverse transcriptaion polymerase chain reaction （RT-PCR）, the expression of MMP2, MMP9 and TIMP2 mRNA in 36 cases of bladder transitional carcinoma and 5 cases of normal bladder mucosa was detected. Results With the increases of tumor grades, the expression of MMP2 mRNA and MMP9 mRNA was significantly increased [x^- （MMP9） G1 ： 1. 218, G2 ： 1. 681, G3 ： 1.811 ; P 〈 0.01 ; T2-T4 ： 1. 840, Tis-T1 ： 1. 399 ; P〈 0.01 ; （MMP2） G1 ： 1. 323, G2：1.694, G3：2. 060;T2-T4-1.950, Tis-T1 ： 1. 505; P〈 0.01],markedly higher than that in normal bladder tissue （x^-： MMP9： 0.455, MMP2： 0.461; P〈 0.01）. With the increase of tumor grades, the expression of TIMP2 mRNA was significantly reduced （x^- ： G1：1.489, Cn：1.391, G3：0.580;P〈0.01）.Conelusion The overexpression of MMP9 and MMP2 mRNA was positively correlated with the malignant degree of BTCC, while the expression of TIMP2 mRNA was negatively related with the malignant degree of BTCC.