肿瘤转移相关基因1在人骨肉瘤细胞的表达与转移侵袭的关系

Correlation of expression of metastasis associated-1 and invasion of human osteosarcoma cells in vitro

目的比较肿瘤转移相关基因(MTA1)在人骨肉瘤细胞高低转移株的表达水平,探讨MTA1表达水平与骨肉瘤细胞转移潜能的相关性。方法采用半定量逆转录-聚合酶链反应 (RT-PCR)检测MG-63骨肉瘤细胞高低转移株MTA1的表达情况,用Boyden小室体外侵袭实验检测两株MG-63细胞的体外侵袭力;用脂质体介导的MTA1基因转染MG-63低转移株细胞,通过 RT-PCR检测MTA1的表达;Boyden小室体外侵袭实验检测转染前后细胞侵袭力的变化。结果 RT-PCR结果显示MTA1在MG-63低转移细胞株中表达水平低(1．32),在高转移细胞株中表达水平高(6．27,P<0．05);Boyden小室体外侵袭实验显示MG-63高转移株细胞体外侵袭力强,其穿膜细胞相对百分率为(46．3±2．4)％,低转移株细胞体外侵袭力较弱,其穿膜细胞相对百分率(12．6± 1．1)％,两者差异有统计学意义(P<0．05);转染MTA1基因后,低转移细胞株转移潜能较未转染细胞明显增高。结论 MTA1与人骨肉瘤细胞转移潜能有密切关系。

Objective To compare the expression level of metastasis associated-1 （MTA1） in highly and low metastatic human osteosarcoma cell lines, and to investigate the correlation of expression level of MTA1 with in vitro invasion of osteosarcoma cells. Methods The expression level of MTA1 in two osteosarcoma cell lines was detected by semiquantitative TR-PCR. Boyden chamber invasion assay was used to evaluate the invasive capacity in vitro in two osteosarcoma cell lines. The low metastatic MG- 63 cell lines were transfected with MTA1 full-length cDNA expression plasmid by lipofectamine. The changes of MTA1 expression and in vitro invasion potential were measured after transfection. Results Highly metastatic MG63 cell line expressed significantly higher level of MTA1 than that of low metastatic MG63 cell by RT-PCR. The invasive potentiality of low metastatic MG63 cell line was increased after MTA1 gene transfection. Conclusion There may be a relationship between MTA1 and invasive potentiality of human osteosarcoma cells.