梗阻性黄疸大鼠血液中内毒素含量与组织形态学变化

The whole blood endotoxin and the histomorphological changes caused by obstructive jaundice in rats

[目的]探讨梗阻性黄疸大鼠血液中内毒素含量与肝及小肠黏膜的形态学变化.[方法]监测假手术组、梗阻性黄疸1,2周组小鼠血胆红素及内毒素含量,采用光学显微镜及电子显微镜观察不同时期梗阻性黄疸大鼠肝及小肠黏膜的形态学变化.[结果]梗阻性黄疸1,2周组大鼠血胆红素及内毒素含量明显升高,与假手术组比较有显著性差异,且肝组织损伤随梗阻时间的延长而逐渐加重,但肠道黏膜的损伤则较轻微.[结论]发生梗阻性黄疸时内毒素血症的发生和发展与肠黏膜的屏障功能密切相关.

OBJECTIVE To study the the content of plasma endotoxin and the histomorphological changes of liver and small intestinal membrane caused by obstructive jaundice in rats. METHODS After executing rats in obstructive jaundice groups for 1, 2 weeks and pseudo operative group with bleeding, the content of serum bilirubin and endotoxin was monitored and the histomorphological transformation of the liver and the small intestine was observed in different period of obstructive jaundice by light microscope and electron microscope. RESULTS The content of serum bilirubin and endotoxin in the obstructive jaundice groups for 1, 2 weeks was obviously increased, respectively, and there was significant difference compared with pseudo operative group. The damage of liver was becoming heavier while the obstruction was prolonged, but the damage of the small intestinal membrane was much slighter. CONCLUSION When happening obstructive jaundice, the occurring and the development of endotoxemia is closely correlated with the protective barrier function of small intestinal membrane in rats.