肿瘤坏死因子单克隆抗体及肝细胞生长多肽对实验性肝坏死的保护

Prevention of experimental hepatic necrosis by hepatopoietin and monoclonal antibody to TNF

为了评估肿瘤坏死因子单抗（ｍＡｂ－ＴＮＦ）及肝细胞生长多肽（ＨＰＮ）的抗肝坏死效果，我们使用大肠杆菌内毒素和鸭乙型肝炎病毒（ＤＨＢＶ）联合诱导建立了鸭急性肝坏死模型。实验结果显示：ｍＡｂ－ＴＮＦ组及ＨＰＮ组血清肿瘤坏死因子活性水平明显低于对照组（Ｐ＜０．０１），且治疗组肝坏死程度也明显减轻（Ｐ＜０．０１）。此外，实验发现血清肿瘤坏死因子活性水平的高低与其急性肝坏死程度成正相关关系（ｒ’ｓ＝０．９０２７，Ｐ＜０．０１）。因此认为，ＨＰＮ是一种阻止实验性急性肝坏死的活性多肽，它的早期使用可抑制机体肿瘤坏死因子的释放。

This study is designed to evaluate the efficacy of monoclonal antibody to TNF (mAb-TNF)and hepatopoietin (HPN)in preventing experimental liver necrosis induced by duck hepatitis B virus(DHBV)combined with lipopolysaccharide(LPS).Both the mAb-TNF and HPN groups displayed significantly lower levels of TNF activity in serum compared with the control group (P<0.01).In addtion,the degree of necrosis in the treated groups decreased significantly(P<0.01).Furthermore,the serum TNF activity was proportionaI to liver necrosis(r’s=0.7 P<0.01).We conclude that HPN is an effective peptide in prevention of acute liver necrosis if given at ap-propriate stage of the disease and that the early use of this peptide may inhibit release of TNF in vi-vo.