流动注射-化学发光法测定药物与体液中的左氧氟沙星

Flow-injection Chemiluminescence Detection for the Determination of Levofloxacin in Pharmaceutical Preparations and Body Fluid

提出了在酸性条件下左氧氟沙星-Ce（Ⅳ）．Na2S2O4化学发光新体系，探讨了该化学发光体系的发光机理．建立了测定左氧氟沙星的流动注射化学发光分析新方法．方法的检出限为7×10^-9g／mL，线性范围为2×10^-8～6×10^-6g／mL，11次平行测定质量浓度为6×10^-7g／mL的左氧氟沙星．其相对标准偏差为2．0％．测定了左氧氟沙星注射液和片剂及血清和尿液中左氧氟沙星的含量，回收率为98．4％～103．7％．结果令人满意．

A new chemiluminescence system, levofloxacin-Ce（Ⅳ ）-Na2S2O4-H2SO4, was developed for the determination of levofloxacin. The chemiluminescence mechanism of the system was discussed. A new chemiluminescence detection method combined with flow injection was described for the determination of levifloxacin in body fluid and pharmaceutical preparations, The detection limit for levofloxacin is 7 × 10^-9 g/mL, and the relative standard deviation （n = 11） is 2.0% at 6 × 10^-7 g/mL. The linear range is 2 × 10^- 8-6 × 10^- 6 g/mL. The method has been applied to the determination of levofloxacin in body fluid and pharmaceutical preparations ,with satisfactory results.