κ-卡拉胶与魔芋粉混合载体固定酵母细胞生物合成谷胱甘肽

Glutathione Biosynthesis by the Immobilized Yeast Cell with κ-Carrgeenin-Konjac Flour

提出了κ-卡拉胶与魔芋粉混合载体固定化微生物细胞的技术,确立了较好的凝胶条件.并利用该混合载体固定酵母细胞进行生物合成谷胱甘肽的研究.实验结果表明:反应液pH值7.0,反应温度37℃,反应时间6 h,磷酸盐缓冲液0.1%的条件下,固定化细胞具有较高的GSH合成酶活力.

A new method for immobilizing microbial cells with κ- carrgeenin - konjac flour was presented and the better gelation conditions were obtained. The study on glutathione biosynthesis by the immobilized yeast cell with κ -carrgeenin - konjac flour was done. The results showed that the better immobilization conditions were as follows ： reactive solution pH 7.0, reactive temperature 37 ℃, reactive time 6 h, potassium phosphate buffer 0.1%.