冠心病中医证型与GNB3基因C825T多态性的关联性分析

Study on the association between traditional Chinese medicine syndrome of Coronary heart disease and polymorphism of C825T of GNB3 gene

目的:探讨冠心病(CHD)中医证型中的血瘀证、痰浊证、非痰非瘀证与G蛋白β3亚单位基因多态性发病和病情程度的相关性。方法:用PCR方法检测22例血瘀证、23例痰浊证、22例非血瘀证冠心病患者及20名正常人的GNB3基因型,同时检测血压、血脂、体重指数等指标。结果:冠心病痰浊证、血瘀证与GNB3基因825TT基因型相关,与其它证型及健康人对照组比较差异显著(P<0.05);痰浊证与血瘀证比较,痰浊证TT基因型携带率高于血瘀型,但是两者比较无统计学差异(P>0.05)。结论:GNB3基因825TT基因型可能是冠心病血瘀证、痰浊证的易感基因。

Objective： To cxplore the relationship between the traditional Chinese medicine syndrome including phlegm damp type （PDS）, blood stasis type （BSS） and the syndrome type caused by neither phegm damp nor blood stasis of coronary heart disease （CHD） and polymorphism of C82.5T of G-protein β3 submit （GNB3） gene,Methods： The GNB3 gene type in 23 patients of CHD of PDS type, 22 patients of CHD of BSS type, 22 patients of CHD of non-PDS and non-BSS type, 20 patients of healthy subjects （control） was determined by PCR assay, also levels of blood pressure, blood fat and BMI was determined. Results： Occurance of 825TT gene type of GNB3 was assoeiated with patients of PDS and BSS.The TT gene type of GNB3 was significantly higher in patients of PDS and BSS than that in other syndrome and control （ P 〈 0.05） .Not only the TT gene type in PDS was higher than that in BSS, but also the difference was not significant （ P 〉 0.05） .Conclusions： 825TT gene type of GNB3 may be a risk element of CHD in patients of PDS and BSS.