摘要
为了寻求肾综合征出血热(HFRS)早期、快速的诊断方法,对196例HFRS患者尿液融合细胞(FC)和血清特异性IgM抗体进行了检测,阳性率分别为85.2%(167/196)和93.9%(184/196),两者差异无显著性(P>0.9)。以ELISAIgM抗体捕捉法为标准考核FC检测的诊断价值,结果FC的灵敏度是ELISA的89.7%;特异度是83.3%;阳性预告值为98.8%;二者的诊断符合率为89.3%。同一时期门诊的麻疹、水痘、风疹、流行性腮腺炎、乙型脑炎等患者尿FC阳性率(13.9%、20%、2.9%、0、0)与HFRS患者的相比差异有显著性(P<0.005)。本文结果提示:FC检测在一定程度上可以取代条件要求较高,操作比较复杂的ELISA。
In order to pursue a rapid diagnostic method for hemorrhagic fever with renal syn-drome(HFRS),we detected the fusion cells(FC)from the urine of 196 patients who were suspectedof contracting HFRS.At the same time,ELISA-IgM antibody-capture assay was used for the detec-tion of IgM against HFRS virus in their sera. The results showed that the positive rate of FC was85.2%(167/196)and the antibody IgM was 93.9%(184/196).By Chi-square test(χ2),there wasno significance between these two groups(P>0.9).Compared to ELISA,the sensitivity of FC de-tection was 89.7%,the specificity was 83.3%;with the positive predicative value of 98.8%and thediagnostic accordance rate of 89.2%.The positive rate of FC detected in HFRS patients was moresignificant than that in patients with measles,chickenpox,German measles,mumps,epidemic en-cephalitis B etc.(P<0.005)This indicated that the detection of FC in the urine from HFRS pa-tients might be used to replace the ELISA-IgM antibody-capture assay for diagnosis of HFRS at theearly stage of the disease, especially in endemic areas(e.g.countryside)far from cities.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1996年第2期89-91,共3页
Chinese Journal of Infectious Diseases