胆管癌组织中P_(21)、P_(53)、bcl-2癌基因蛋白的表达及生物学意义

The expressions of P21, P53 and Bcl-2 on-coproteins in cholangiocarcinoma tissue and their biological significance

应用免疫组化方法对42例胆管癌组织进行P_(21)、P_(53)及bcl-2癌基因蛋白检测并探讨其生物学意义。结果发现:三种癌基因蛋白的阳性表达率分别为52.4%、47.6%、57.1%;bcl-2蛋白与P_(53)蛋白的表达呈明显的负相关,未分化癌P_(53)蛋白阳性率高于高分化和低分化腺癌(P<0.05),高分化腺癌bcl-2蛋白阳性率高于低分化和未分化癌(P<0.05);三种癌基因蛋白的表达与胆管癌临床特征无明显相关性。结果表明;(1)P_(21)、P_(53)及bcl-2癌基因蛋白在胆管癌组织中有较高表达率;(2)P_(53)蛋白阳性的病例恶性程度高、预后较差,而bcl-2蛋白阳性的病例则相反,两者可能是反映胆管癌生物学行为和预后的重要标记物,在胆管癌发生发展中可能有重要意义。

The expression of oncoproteins including P21, P53 and Bcl-2) were investigated by ABC immunohistochemical method in 42 cases of cholangiocarcinoma. The positive expression rates of P21, P53 and Bcl-2 were 52.4%, 47.6% and 57.1% respectively. The negative correlation was found between the expression of Bcl-2 and P53. The positive rate of P53 in undifferentiated carcinomas was higher that in well-differentiated or poorlydifferentiated adenocarcinomas(P<0.05); the positive rate of Bcl-2 in well-differentiated adenocarcinomas was higher than the poorlydifferentiated or undifferentiated carcinomas (P<0.05). The close correlation were not found among the expression of oncoproteins and the clinical features. These results suggested that: 1) Higher expression rates of P21, P53 and Bcl-2 were found in cholangiocarcinoma; (2) The positive case with P53 might be highly malignant with poorly-prognosis; the positive cases with Bcl-2 might have a low malignant degree and good-prognosis. The P53, Bcl-2 might be important markers in reflecting the biological behaviors and prognosis of cholangiocarcinomas and might play an important role in the development of cholangiocarcinomas.