弥漫型肾间质纤维化小鼠模型的快速制备及实验研究

Preliminary observation on diffuse tubulointerstitial fibrosis rapidly induced in mice by unilateral ureteral obstruction (UUO)

本文应用单侧输尿管便阻的方法制备小鼠肾间质纤维化模型。小鼠输尿管梗阻后１５ｄ，肾小管上皮细胞出现大量坏死，间质炎细胞浸润，肾间质面积增宽；术后２１ｄ，肾小管和间质的结构基本消失，肾间质呈弥漫性细胞增殖；２４ｄ，肾间质中增殖的细胞数量减少，出现广泛的纤维化。用免疫组化法证实肾间质中增殖的细胞是成纤维细胞。本模型的建立为研究肾间质纤维化的发生发展提供了一种快速。

Experimental diffuse tubulointerstitial fibrosis was rapidly induced by UUO in mice. By the 15th day of UUo, the tubulointerstitium showed extensive epithelial cell necrosis, inflammatory cell infiltration and interstitial volume expansion. By the 18th day of UUO, focal cellular proliferation occured in renal interstitium and then replaced by extensive cellular proliferation at the 21st day of UUO, By the 24th day of UUO, the renal interstitium showed diffuse fibrosis. These proliferative cells in renal interstitium were identified as fibroblasts by immunohistochemical stain. The result suggested that fibroblasts may play an important role in tubulointerstitial fibrogenesis.